Difference between revisions of "Part:BBa K2719002:Experience"

Revision as of 12:08, 17 October 2018

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Applications of BBa_K2719002

To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 2)

Figure 1. Transformed Tenascin 5 Domain V colonies

To prove the presence of the plasmid in E. coli it was necessary to create an agarose gel. Because polyproline linker wasn’t binded in this fusion protein, the bands presented in the gel were less heavy. (Figure 2).

After that, another agarose gel was made for the restriction parts to confirm the presence of the insert (figure 3).

User Reviews

UNIQ68b01b0df95d30cc-partinfo-00000000-QINU UNIQ68b01b0df95d30cc-partinfo-00000001-QINU

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 ===Applications of BBa_K2719002===
 <p>To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in <i>Escherichia coli</i> DH5a. (Figure 2)</p>
-[[file:T--TecCEM--TCD5Colonies.png]]
+[[file:T--TecCEM--TCD5Colonies.png|500px]]
 <p><i>Figure 1.</i> Transformed Tenascin 5 Domain V colonies</p>
 <p>To prove the presence of the plasmid in <i>E. coli</i> it was necessary to create an agarose gel. Because polyproline linker wasn’t binded in this fusion protein, the bands presented in the gel were less heavy. (Figure 2).  </p>