Difference between revisions of "Part:BBa K209496"

 
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<partinfo>BBa_K209496 parameters</partinfo>
 
<partinfo>BBa_K209496 parameters</partinfo>
 
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===2018 Peking Team improvement===
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Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.
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Firstly, we fused this this part with yEGFP, which made it visible in the yeast through fluorescent microscope.
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https://parts.igem.org/Part:BBa_K2601007
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Then we characterized the expression of Frb-yEGFP with 2 promoters pTet07 and PDH3. The expression of these promoters was measured through flow cytometry.
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https://parts.igem.org/Part:BBa_K2601021;
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https://parts.igem.org/Part:BBa_K2601023
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Moreover, we fused Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.
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https://parts.igem.org/Part:BBa_K2601010
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Finally, we characterized the expression of Frb-yEGFP-HOTag6 with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.
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https://parts.igem.org/Part:BBa_K2601032;
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https://parts.igem.org/Part:BBa_K2601033;
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https://parts.igem.org/Part:BBa_K2601034

Latest revision as of 11:59, 17 October 2018


AarI B-D part, FRB



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



2018 Peking Team improvement

Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.

Firstly, we fused this this part with yEGFP, which made it visible in the yeast through fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601007

Then we characterized the expression of Frb-yEGFP with 2 promoters pTet07 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601021; https://parts.igem.org/Part:BBa_K2601023


Moreover, we fused Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601010

Finally, we characterized the expression of Frb-yEGFP-HOTag6 with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601032; https://parts.igem.org/Part:BBa_K2601033; https://parts.igem.org/Part:BBa_K2601034