Difference between revisions of "Part:BBa K2839000"

Revision as of 11:11, 17 October 2018

TALEsp1-Pupsp1 stabilized promoter

TALE1 stabilized promoter driving sfGFP expression

Talesp1 pupsp1 Stabilized Device

Contents: Short Description Biology and Functionality Usage in our Project Illegal Sites Removal Cloning Strategy Results, Characterization Potential Applications References

1.Short description:

This part consists of a TAL Effector (TALEsp1), a TALEsp1-stabilized promoter (pupsp1) and a fluorescent marker (sfGFP). This part was originally designed by Thomas Hale Segall-Shapiro (Shapiro et al) and iGEM Thessaloniki modified it to exclude 2 PstI recognition sites, thus making it RFC[10] compatible .It can be used to achieve stabilised expression of the sfGFP marker, decoupled from gene/plasmid copy number.

Basic Part Name Registry link Type Function L2S2P21 BBa_K1401006 Terminator Terminates Transcription PT7A1w2 BBa_K2839021 Promoter Transcription Initiation SarJ BBa_K2839022 Insulator

RBSsp1 BBa_K2753036 RBS Recognized by ribosome and initiates translation TALEsp1 BBa_K2839027 Coding Sequence Binds to the PUPsp1 promoter and inhibits RNAP binding ECK-12 0029600 BBa_K2486006 Terminator Terminates Transcription PUPsp1 BBa_K2839025 Promoter Transcription Initiation RiboJ BBa_K1679042 Insulator

B0032 BBa_B0032 RBS Ribosome Binding Site sfGFP BBa_K1893032 Coding Sequence Fluorescence Marker B0015 BBa_B0015 Terminator Terminates Transcription

Sequence and Features