Difference between revisions of "Part:BBa K209023"

 
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<partinfo>BBa_K209023 parameters</partinfo>
 
<partinfo>BBa_K209023 parameters</partinfo>
 
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===2018 Peking Team improvement===
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Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.
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Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.
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https://parts.igem.org/Part:BBa_K2601008
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Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.
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https://parts.igem.org/Part:BBa_K2601025;
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https://parts.igem.org/Part:BBa_K2601026;
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https://parts.igem.org/Part:BBa_K2601027
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Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
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https://parts.igem.org/Part:BBa_K2601011
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Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.
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https://parts.igem.org/Part:BBa_K2601037;
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https://parts.igem.org/Part:BBa_K2601038

Latest revision as of 11:00, 17 October 2018


Aar1 C-D part, FKBP

dimerizes with FRB when rapamycin is present

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2018 Peking Team improvement

Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.

Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601008

Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601025; https://parts.igem.org/Part:BBa_K2601026; https://parts.igem.org/Part:BBa_K2601027

Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601011

Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.

https://parts.igem.org/Part:BBa_K2601037; https://parts.igem.org/Part:BBa_K2601038