Difference between revisions of "Part:BBa K209023"
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===2018 Peking Team improvement=== | ===2018 Peking Team improvement=== | ||
| − | Team Peking 2018 improved this part. | + | Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details. |
Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope. | Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope. | ||
| Line 27: | Line 27: | ||
Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry. | Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry. | ||
| − | https://parts.igem.org/Part:BBa_K2601025 | + | https://parts.igem.org/Part:BBa_K2601025; |
| − | https://parts.igem.org/Part:BBa_K2601026 | + | https://parts.igem.org/Part:BBa_K2601026; |
https://parts.igem.org/Part:BBa_K2601027 | https://parts.igem.org/Part:BBa_K2601027 | ||
| Line 37: | Line 37: | ||
Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry. | Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry. | ||
| − | https://parts.igem.org/Part:BBa_K2601037 | + | https://parts.igem.org/Part:BBa_K2601037; |
https://parts.igem.org/Part:BBa_K2601038 | https://parts.igem.org/Part:BBa_K2601038 | ||
Latest revision as of 11:00, 17 October 2018
Aar1 C-D part, FKBP
dimerizes with FRB when rapamycin is present
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2018 Peking Team improvement
Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.
Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.
https://parts.igem.org/Part:BBa_K2601008
Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.
https://parts.igem.org/Part:BBa_K2601025; https://parts.igem.org/Part:BBa_K2601026; https://parts.igem.org/Part:BBa_K2601027
Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
https://parts.igem.org/Part:BBa_K2601011
Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.
https://parts.igem.org/Part:BBa_K2601037; https://parts.igem.org/Part:BBa_K2601038