Difference between revisions of "Part:BBa K2622003"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2622003 short</partinfo> | <partinfo>BBa_K2622003 short</partinfo> | ||
| − | + | Translocator domain from Neisseria gonorrhoeae (1087-1532 aa.) forms a β-barrel inside an outer membrane of Gram- bacteria. It has an α-helix forming linker in N-terminus, which passes through the β-barrel to the outside of the bacteria. Fused protein particles to the N-terminus of the translocator domain will be displayed on the surface of Gram- bacteria. Therefore, this biobrick has RFC12 prefix and suffix that allows to fuse proteins to the N or C-terminus in a stop codon free way. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 10:14, 17 October 2018
Immunoglobulin A-serine specific endopeptidase's translocator domain
Translocator domain from Neisseria gonorrhoeae (1087-1532 aa.) forms a β-barrel inside an outer membrane of Gram- bacteria. It has an α-helix forming linker in N-terminus, which passes through the β-barrel to the outside of the bacteria. Fused protein particles to the N-terminus of the translocator domain will be displayed on the surface of Gram- bacteria. Therefore, this biobrick has RFC12 prefix and suffix that allows to fuse proteins to the N or C-terminus in a stop codon free way.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal AgeI site found at 673 - 1000COMPATIBLE WITH RFC[1000]