Difference between revisions of "Part:BBa K2684000"
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Revision as of 09:47, 17 October 2018
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Period - CsgA - SpyTag
Reaction stock leftover in experiment
Using sfGFP – spycatcher protein, the combing function of Spytag and spycatcher system on the biofilm was tested. Gene csgA on the plasmid of pET28a was transferred in to ΔMG1655 as control group. Gene csgA – Spytag on the plasmid of pET28a was transferred in to ΔMG1655 as experiment. To verify the function of Spytag on csgA, the experiment was design to compare the combing rate of sf-GFP – spycatcher protein with cells that have csgA – SpyTag (Experiment) or csgA (Control).
As can be seen from the result,
Thus we can confirm our csgA – SpyTag system is functional.