Difference between revisions of "Part:BBa K2588022"
 
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<h2>Usage and Biology</h2>
 
<h2>Usage and Biology</h2>
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<p>This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 unsaturated fatty acid (myristic acid) as seen in fig. 1.<sup>1</sup> The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occurs as an α<sub>2</sub>β<sub>2</sub> heterotetramer.<sup>2,3</sup>
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<p>This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 saturated fatty acid (myristic acid) as seen in fig. 1.<sup>1</sup> The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occur as an α<sub>2</sub>β<sub>2</sub> heterotetramer.<sup>2,3</sup>
 
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<img src="https://static.igem.org/mediawiki/parts/b/b3/Fattyacidssynthesisecoli.png" style="width: 100%">
 
<img src="https://static.igem.org/mediawiki/parts/b/b3/Fattyacidssynthesisecoli.png" style="width: 100%">
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<center><p>Fig. 1 (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.<sup>1</sup></p></center>
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<center><p>Fig. 1: (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.<sup>1</sup></p></center>
  
 
<h2>References</h2>
 
<h2>References</h2>

Latest revision as of 09:47, 17 October 2018


myristic acid synthesis module, glucose-induced

Usage and Biology

This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 saturated fatty acid (myristic acid) as seen in fig. 1.1 The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occur as an α2β2 heterotetramer.2,3

Fig. 1: (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.1

References

  1. Xu, P. et al. Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat. Commun. 4, 1409 (2013).
  2. Li, S. J. & Cronan, J. E. The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase. J. Biol. Chem. 267, 855–63 (1992).
  3. Broussard, T. C., Price, A. E., Laborde, S. M. & Waldrop, G. L. Complex formation and regulation of escherichia coli acetyl-CoA carboxylase. Biochemistry (2013). doi:10.1021/bi4000707


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2317
    Illegal AgeI site found at 1097
    Illegal AgeI site found at 2851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1035
    Illegal SapI.rc site found at 1232