Difference between revisions of "Part:BBa K2542007"

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We placed the homology arms at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful.
 
We placed the homology arms at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful.
 
[[http://2018.igem.org/Team:NEFU_China/Lock_Key See more details]]  
 
[[http://2018.igem.org/Team:NEFU_China/Lock_Key See more details]]  
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[[Image:T--NEFU_China--homologous_recombination_PCR.jpg|thumb|560px]]
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[[Image:T--NEFU_China--homologous_recombination_PCR.jpg|thumb|350px]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 08:22, 17 October 2018


Part of the transposable element of Saccharomyces cerevisiae.

It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is BBa_K2542008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiment

We placed the homology arms at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful. http://2018.igem.org/Team:NEFU_China/Lock_Key See more details

T--NEFU China--homologous recombination PCR.jpg