Difference between revisions of "Part:BBa K2789021:Design"
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Start with ATG and end with TAA, this is a standard coding sequence and it worked well in our project.You can use this part to build more complicated pathway! | Start with ATG and end with TAA, this is a standard coding sequence and it worked well in our project.You can use this part to build more complicated pathway! | ||
| + | Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer. <br> | ||
| + | fwd: 5'-GCGGCCGC T TCTAG ATGGTTGTTGTTGGTAAATCTG-3' <br> | ||
| + | rev: 5'-GTTTCTTC CTGCAG CGGCCGC T ACTAGT A TGCGGCCGCTTAGTCG-3' | ||
Latest revision as of 08:14, 17 October 2018
PPN(endopolyphosphotase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 315
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1965
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Start with ATG and end with TAA, this is a standard coding sequence and it worked well in our project.You can use this part to build more complicated pathway!
Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-GCGGCCGC T TCTAG ATGGTTGTTGTTGGTAAATCTG-3'
rev: 5'-GTTTCTTC CTGCAG CGGCCGC T ACTAGT A TGCGGCCGCTTAGTCG-3'
Source
This part comes from the genome of Saccharomyces cerevisiae and we carry out codon optimization for sequences to make it express best in E.coli.BL21.