Difference between revisions of "Part:BBa K2788020:Design"

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===Design Notes===
 
===Design Notes===
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In order to verify that TupA was indeed expressed at the protein level, 2018SZU-China added a 6-His structure to the original sequence and verified it by western blot.
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In order to analyze TupA at the protein level, 2018SZU-China added a his-tag to the C terminal and verified it by histidine affinity chromatography and SDS-PAGE.
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===Source===
 
===Source===

Latest revision as of 07:58, 17 October 2018


C125-TupA-6His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 789
    Illegal BsaI.rc site found at 1181


Design Notes

In order to analyze TupA at the protein level, 2018SZU-China added a his-tag to the C terminal and verified it by histidine affinity chromatography and SDS-PAGE.

Source

BBa_K2232003

References

This part created by 2018 SZU-China iGEM team basing on the original part:BBa_K2232003

Aono R, Ito M, Machida T. Contribution of the Cell Wall Component Teichuronopeptide, to pH Homeostasis and Alkaliphily in the Alkaliphile, Bacillus lentus C-125[J]. Journal of Bacteriology, 1999, 181(21):6600-6.