Difference between revisions of "Part:BBa K2611004:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The sgRNA sequence is generated by a special algorithm. And the backbone is BBa_K1689000.
+
The sgRNA sequence is selected by BLAST all spacer sequence generated by GFP open reading frame with the genome of E. coli BL21 strain. And the backbone is BBa_K1689000.
  
 
===References===
 
===References===

Latest revision as of 07:29, 17 October 2018


sgRNA (J23100)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Should carefully notice the complementary of the sgRNA and its target sequence(J23100 constitutive promoter).


Source

The sgRNA sequence is selected by BLAST all spacer sequence generated by GFP open reading frame with the genome of E. coli BL21 strain. And the backbone is BBa_K1689000.

References