Difference between revisions of "Part:BBa K2588022"
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<html> <p>This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 unsaturated fatty acid (myristic acid) as seen in fig. 1.<sup>1</sup> The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occurs as an α<sub>2</sub>β<sub>2</sub> heterotetramer.<sup>2,3</sup>
 
<html> <p>This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 unsaturated fatty acid (myristic acid) as seen in fig. 1.<sup>1</sup> The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occurs as an α<sub>2</sub>β<sub>2</sub> heterotetramer.<sup>2,3</sup>
 
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<img src="https://static.igem.org/mediawiki/parts/b/b3/Fattyacidssynthesisecoli.png">
 
<p>Fig. 1 (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.<sup>1</sup>
 
<p>Fig. 1 (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.<sup>1</sup>
 
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Revision as of 07:23, 17 October 2018


myristic acid synthesis module, glucose-induced

This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 unsaturated fatty acid (myristic acid) as seen in fig. 1.1 The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occurs as an α2β2 heterotetramer.2,3

Fig. 1 (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal NotI site found at 1841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2317
    Illegal AgeI site found at 1097
    Illegal AgeI site found at 2851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1035
    Illegal SapI.rc site found at 1232