Difference between revisions of "Part:BBa K2789001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 07:17, 17 October 2018
lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator
DNA element : 1.Ccar and Ccas : They could form heterodimer as transcription factor to activate the Cpcg (light control promotor) under green light. And the heterodimer is unstable under blue light.
2.Lac operator : They could block the expression of downstream genes and regain the promotor ability after inducted by IPTG
3.Cpcg : The promotor could be activated by heterodimer made up of Ccar and Ccas
4.TetR : TetR could bind to the tetracydine operator tetO to inhibit transcription .
5.TetO : TetO could be inhibited by tetR
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1393
Illegal BamHI site found at 2973 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 798
Illegal AgeI site found at 1803
Illegal AgeI site found at 1998 - 1000COMPATIBLE WITH RFC[1000]