Difference between revisions of "Part:BBa K2876005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The part needed a 3-Alanine linker. It was codon optimized for E. coli. | |
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===Source=== | ===Source=== | ||
Latest revision as of 07:14, 17 October 2018
A fusion of a RNAPolIII alpha subunit and antiAFB1-ScFv1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 8
Illegal NotI site found at 766 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 480
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part needed a 3-Alanine linker. It was codon optimized for E. coli.
Source
Part:BBa_K2247007, BBa_K2876002, BBa_K2876004
References
Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0
Li, X., Li, P. and Zhang, Q., Molecular analysis of monoclonal antibodies against aflatoxins: a prediction of functional amino acid which influences antibody affinity. Chinese Academy of Agricultural Sciences, 2011.
Pei, S.a.S., D., Construction and identification of mouse Anti-AFB1 phage single-chain antibody library. Food and Biological Engineering College, 2010.