Difference between revisions of "Part:BBa K2876005"
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<partinfo>BBa_K2876005 short</partinfo> | <partinfo>BBa_K2876005 short</partinfo> | ||
| − | A fusion of a RNAPolIII alpha subunit and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv1) for an E. coli two hybrid system | + | A fusion of a RNAPolIII alpha subunit and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv1). Used in conjunction with BBa_K2876003 (https://parts.igem.org/Part:BBa_K2876005) and BBa_K2876000 (https://parts.igem.org/Part:BBa_K2876000) to create for an E. coli two hybrid system capable of detecting aflatoxin. |
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Revision as of 07:14, 17 October 2018
A fusion of a RNAPolIII alpha subunit and antiAFB1-ScFv1
A fusion of a RNAPolIII alpha subunit and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv1). Used in conjunction with BBa_K2876003 (https://parts.igem.org/Part:BBa_K2876005) and BBa_K2876000 (https://parts.igem.org/Part:BBa_K2876000) to create for an E. coli two hybrid system capable of detecting aflatoxin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 8
Illegal NotI site found at 766 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 480
- 1000COMPATIBLE WITH RFC[1000]