Difference between revisions of "Part:BBa K2876002:Design"

 
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===Design Notes===
 
===Design Notes===
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codon optimized for E. coli
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codon optimized for E. coli, with the additional constraint of codon optimization to remove restriction sites allowing for pSB1C3 compatibility.
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===Source===
 
===Source===
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===References===
 
===References===
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Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0

Latest revision as of 06:58, 17 October 2018


RNAPolIII alpha subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 8
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

codon optimized for E. coli, with the additional constraint of codon optimization to remove restriction sites allowing for pSB1C3 compatibility.

Source

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0

References

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0