Difference between revisions of "Part:BBa K2611003:Design"

Latest revision as of 06:52, 17 October 2018

Spacer J23101-RFP

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 30
Illegal NheI site found at 53
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 640
Illegal AgeI site found at 752
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The spacer sequence and the NGG site should be closely to the promoter (no space in this part).

Source

The spacer sequence is generated by a special algorithm.[1] And the backbone we used is from BBa_K516132. [1]Didovyk A, Borek B, Hasty J, et al. Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9[J]. Acs Synthetic Biology, 2016, 5(1):81-88.

References

[1]Didovyk A, Borek B, Hasty J, et al. Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9[J]. Acs Synthetic Biology, 2016, 5(1):81-88.

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	===References===		===References===
		+	[1]Didovyk A, Borek B, Hasty J, et al. Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9[J]. Acs Synthetic Biology, 2016, 5(1):81-88.