Difference between revisions of "Part:BBa K2705006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | It was applied to sieve Bacillus amyloliquefaciens LL3 bacteria with high level glutamate under tetracycline circumstance, which can produce more products whose precursor is glutamate such as γ-PGA. Since LL3 can produce glutamate by itself, high-producing cells can survive based on the amount of precursor. | + | It was applied to sieve <i>Bacillus amyloliquefaciens</i> LL3 bacteria with high level glutamate under tetracycline circumstance, which can produce more products whose precursor is glutamate such as γ-PGA. Since LL3 can produce glutamate by itself, high-producing cells can survive based on the amount of precursor. |
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===Source=== | ===Source=== | ||
| − | + | P<sub>''gltAB''</sub> was acqcuired from LL3 by PCR, LacI was from vector pTH01 by PCR, P<sub><i>grac</i></sub> was from vector pTH01 by PCR, TetA was from vector pWH1520 by PCR. | |
===References=== | ===References=== | ||
Latest revision as of 06:16, 17 October 2018
PgltAB-LacI-Pgrac-TetA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1229
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was applied to sieve Bacillus amyloliquefaciens LL3 bacteria with high level glutamate under tetracycline circumstance, which can produce more products whose precursor is glutamate such as γ-PGA. Since LL3 can produce glutamate by itself, high-producing cells can survive based on the amount of precursor.
Source
PgltAB was acqcuired from LL3 by PCR, LacI was from vector pTH01 by PCR, Pgrac was from vector pTH01 by PCR, TetA was from vector pWH1520 by PCR.