Difference between revisions of "Part:BBa K2662002"
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<partinfo>BBa_K2662002 short</partinfo>
 
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Codon-optimized for E. coli 7-Epi-Sesquithujene Synthase, is derived from Terpene Synthase 4 from Zea mays cultivar B73, catalyzes farnesyl diphosphate to 7-epi-sesquithujene and other sesquiterpenoids.
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This gene encodes for 7-epi-sesquithujene synthase, which is derived from Terpene Synthase 4 from Zea mays cultivar B73. It catalyzes farnesyl pyrophosphate to 7-epi-sesquithujene and other sesquiterpenoids, including beta-bisabolene.  
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===Usage and Biology===
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===Results===
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[[Image:T--UNebraska-Lincoln--results_fig_11_2018.png|450px|thumb|left|'''Figure 1:'''Production of 7-epi-sesquithujene under differing growing conditions.]]Codon-optimized 7-epi-sesquithujene synthase (TPS4CO) was paired with genes encoding the mevalonate-dependent pathway, which produces farnesyl pyrophosphate, in a two plasmid system. The cultures were overlaid with n-octane to partition the sesqueterpenoid products. Octane samples were analyzed through gas chromatography-mass spectrometry to identify and quantify sesqueterpenoid products. The adjacent results show that sesquiterpene production can vary widely with growing conditions, as exampled by the major product 7-epi-sesquithujene.
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[[Image:T--UNebraska-Lincoln--results_fig_14_2018.png|450px|thumb|left|'''Figure 2:''' Production comparison of 7-epi-sesquithujene to beta-bisabolene.]]Six biological replicates were tested to determine if product production was consistent under identical growing conditions. For these replicates, 7-epi-sesquithujene and beta-bisabolene production were quantified. The adjacent results show that sesquiterpenoid production was greatly varied, with observed concentration of the major product ranging between 0.52 to 92.78 mg/L. There must be a variable controlling production that has not been identified yet. In these replicates, it was observed that although production varied, 7-epi-sesquithujene is the major product and beta-bisabolene is the second major product of 7-epi-sesquithujene synthase.
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Revision as of 05:55, 17 October 2018


7-Epi-Sesquithujene Synthase

This gene encodes for 7-epi-sesquithujene synthase, which is derived from Terpene Synthase 4 from Zea mays cultivar B73. It catalyzes farnesyl pyrophosphate to 7-epi-sesquithujene and other sesquiterpenoids, including beta-bisabolene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]