Difference between revisions of "Part:BBa K2683020"
Kristiturton (Talk | contribs) |
Kristiturton (Talk | contribs) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 6: | Line 6: | ||
The part was improved by placing it into the pSB1C3 plasmid. | The part was improved by placing it into the pSB1C3 plasmid. | ||
| + | |||
| + | https://static.igem.org/mediawiki/2018/d/dc/T--Lethbridge--PheRSaOE.PNG | ||
| + | |||
| + | Figure 1- Overexpression of PheRS alpha protein run on a 12% SDS-PAGE. Samples were taken at point of induction and then in 1 hour increments thereafter. The induced PheRSalpha protein can be seen at about 38kDa. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 03:34, 17 October 2018
Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha) in pSB1C3
Phenylalanine tRNA synthetase (PheRS) is responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. PheRS has both a alpha and beta subunit [1]. The part is codon optimized for use in Escherichia coli , contains a N-terminal hexahistidine tag with a serine glycine linker and is under the regulation of a T7 Promoter, medium strength RBS and double terminator. The part was originally designed by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge) and was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443014).
The part was improved by placing it into the pSB1C3 plasmid.
Figure 1- Overexpression of PheRS alpha protein run on a 12% SDS-PAGE. Samples were taken at point of induction and then in 1 hour increments thereafter. The induced PheRSalpha protein can be seen at about 38kDa.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]