Difference between revisions of "Part:BBa K2683019"
 
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This part was improved by LEthbridge iGEM 2018 by placing the part in the pSB1C3 plasmid.  
 
This part was improved by LEthbridge iGEM 2018 by placing the part in the pSB1C3 plasmid.  
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https://static.igem.org/mediawiki/2018/f/f5/T--Lethbridge--LysRSOE.PNG
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Figure 1- Overexpression of LysRS protein run on a 12% SDS-PAGE gel. Samples were taken at point of induction and in 1 hour increments after. The induced samples can be seen at about the 60kDa mark.
  
 
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Latest revision as of 03:28, 17 October 2018


Lysine tRNA Synthetase (LysRS) in pSB1C3

Lysine tRNA synthetase is a protein responsible for attaching lysine onto its tRNA to form an aminoacyl-tRNA [1]. This part is codon optimized for use in Escherichia coli , contains a N-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team in 2017 (http://2017.igem.org/Team:Lethbridge).

This part was improved by LEthbridge iGEM 2018 by placing the part in the pSB1C3 plasmid.

T--Lethbridge--LysRSOE.PNG

Figure 1- Overexpression of LysRS protein run on a 12% SDS-PAGE gel. Samples were taken at point of induction and in 1 hour increments after. The induced samples can be seen at about the 60kDa mark.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1459
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 733