Difference between revisions of "Part:BBa K2683019"

Latest revision as of 03:28, 17 October 2018

Lysine tRNA Synthetase (LysRS) in pSB1C3

Lysine tRNA synthetase is a protein responsible for attaching lysine onto its tRNA to form an aminoacyl-tRNA [1]. This part is codon optimized for use in Escherichia coli , contains a N-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team in 2017 (http://2017.igem.org/Team:Lethbridge).

This part was improved by LEthbridge iGEM 2018 by placing the part in the pSB1C3 plasmid.

Figure 1- Overexpression of LysRS protein run on a 12% SDS-PAGE gel. Samples were taken at point of induction and in 1 hour increments after. The induced samples can be seen at about the 60kDa mark.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1459
Illegal NgoMIV site found at 1531
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 733

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683019 short</partinfo>
-LysRs now in the biobrick standard
+Lysine tRNA synthetase is a protein responsible for attaching lysine onto its tRNA to form an aminoacyl-tRNA [1]. This part is codon optimized for use in <i> Escherichia coli </i>, contains a N-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team in 2017 (http://2017.igem.org/Team:Lethbridge).
+This part was improved by LEthbridge iGEM 2018 by placing the part in the pSB1C3 plasmid.
+https://static.igem.org/mediawiki/2018/f/f5/T--Lethbridge--LysRSOE.PNG
+Figure 1- Overexpression of LysRS protein run on a 12% SDS-PAGE gel. Samples were taken at point of induction and in 1 hour increments after. The induced samples can be seen at about the 60kDa mark.
 <!-- Add more about the biology of this part here