Difference between revisions of "Part:BBa K2683017"
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− | Figure 1- The overexpression of | + | Figure 1- The overexpression of Release Factor 2 in pSb1C3 as run on a 12% SDS-PAGE gel. Samples taken correspond to point of induction and samples taken over the next three hours. |
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 03:07, 17 October 2018
Release Factor 2 in pSB1C3
Release Factor 2 (RF2) is a protein that causes termination of translation by recognizing the stop codon in an mRNA sequence. This part was originally designed by Lethbridge iGEM team 2017(http://2017.igem.org/Team:Lethbridge) The original composite part can be found here: https://parts.igem.org/Part:BBa_K2443030 RF2 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. It is under the regulation of T7 Promoter, RBS and double terminator.
We have improved the part by successfully inserting it into the pSB1C3 standard plasmid.
Figure 1- The overexpression of Release Factor 2 in pSb1C3 as run on a 12% SDS-PAGE gel. Samples taken correspond to point of induction and samples taken over the next three hours. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 659
Illegal BglII site found at 1116 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]