Difference between revisions of "Part:BBa K2680552"
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<partinfo>BBa_K2680552 short</partinfo> | <partinfo>BBa_K2680552 short</partinfo> | ||
| − | "This part codes for the AAA+ Lon Protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006." <sup>1</sup> This Mf-Lon is identical to part Part:BBa_K2333011 but does not include the stop codon. This part also includes an ssrA tag, a degradation tag made from AANDENYALA, which fuses to the C-terminal of proteins for fast degradation through SspB-mediated binding to the ClpX protease | + | "This part codes for the AAA+ Lon Protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006." <sup>1</sup> This Mf-Lon is identical to part Part:BBa_K2333011 but does not include the stop codon. This part also includes an ssrA tag, a degradation tag made from AANDENYALA, which fuses to the C-terminal of proteins for fast degradation through SspB-mediated binding to the ClpX protease in addition to ClpA protease. <sup>2</sup> |
===References=== | ===References=== | ||
1. Jones, E. (2017, October 27). Part:BBa_K2333011. Retrieved from https://parts.igem.org/Part:BBa_K2333011 | 1. Jones, E. (2017, October 27). Part:BBa_K2333011. Retrieved from https://parts.igem.org/Part:BBa_K2333011 | ||
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2. McGinness, K. E., Baker, T., & Sauer, R. (2006, June 06). Engineering Controllable Protein Degradation. Retrieved from https://www.sciencedirect.com/science/article/pii/S1097276506003261?via=ihub | 2. McGinness, K. E., Baker, T., & Sauer, R. (2006, June 06). Engineering Controllable Protein Degradation. Retrieved from https://www.sciencedirect.com/science/article/pii/S1097276506003261?via=ihub | ||
Latest revision as of 02:05, 17 October 2018
Mf-lon SsrA
"This part codes for the AAA+ Lon Protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006." 1 This Mf-Lon is identical to part Part:BBa_K2333011 but does not include the stop codon. This part also includes an ssrA tag, a degradation tag made from AANDENYALA, which fuses to the C-terminal of proteins for fast degradation through SspB-mediated binding to the ClpX protease in addition to ClpA protease. 2
References
1. Jones, E. (2017, October 27). Part:BBa_K2333011. Retrieved from https://parts.igem.org/Part:BBa_K2333011
2. McGinness, K. E., Baker, T., & Sauer, R. (2006, June 06). Engineering Controllable Protein Degradation. Retrieved from https://www.sciencedirect.com/science/article/pii/S1097276506003261?via=ihub
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1834
Illegal AgeI site found at 1918
Illegal AgeI site found at 2124
Illegal AgeI site found at 2149 - 1000COMPATIBLE WITH RFC[1000]