Difference between revisions of "Part:BBa K2812007:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2812007 short</partinfo>
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<partinfo>BBa_K2812006 short</partinfo>
  
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<partinfo>BBa_K2812007 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2812006 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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Optimized for E. coli.
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Original DNA sequence based on ''Pseudomonas aeruginosa'' PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985).<sup>1</sup> This DNA sequence is codon optimized for E. coli. AraC promotor <partinfo>BBa_K2812002</partinfo> was assembled using Gibson assembly.
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===Source===
 
===Source===
  
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...
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Genomic sequence from ''Pseudomonas aeruginosa''. DNA construct produced by IDT.
  
 
===References===
 
===References===
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1) Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." ''FEBS letters'' 584.15 (2010): 3354-3358.

Revision as of 01:41, 17 October 2018


Coding sequence for Pyocin S5 with HlyA and His6-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal BglII site found at 425
    Illegal BglII site found at 2110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1200


Design Notes

Original DNA sequence based on Pseudomonas aeruginosa PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985).1 This DNA sequence is codon optimized for E. coli. AraC promotor BBa_K2812002 was assembled using Gibson assembly.

Source

Genomic sequence from Pseudomonas aeruginosa. DNA construct produced by IDT.

References

1) Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.