Difference between revisions of "Part:BBa K2680278"
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3G mf-Lon SsrA flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. | 3G mf-Lon SsrA flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. | ||
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+ | mf-Lon is a protease that degrades proteins with the ssrA tag. | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 01:31, 17 October 2018
3G mf-Lon SsrA
3G mf-Lon SsrA flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
mf-Lon is a protease that degrades proteins with the ssrA tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1930
Illegal AgeI site found at 2014
Illegal AgeI site found at 2220
Illegal AgeI site found at 2245 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI site found at 89
Illegal BsaI.rc site found at 2502
Illegal BsaI.rc site found at 2553