Difference between revisions of "Part:BBa K2680278"

 
 
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<partinfo>BBa_K2680278 short</partinfo>
 
<partinfo>BBa_K2680278 short</partinfo>
  
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3G mf-Lon SsrA
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3G mf-Lon SsrA flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
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mf-Lon is a protease that degrades proteins with the ssrA tag.
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Latest revision as of 01:31, 17 October 2018


3G mf-Lon SsrA

3G mf-Lon SsrA flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

mf-Lon is a protease that degrades proteins with the ssrA tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1930
    Illegal AgeI site found at 2014
    Illegal AgeI site found at 2220
    Illegal AgeI site found at 2245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI site found at 89
    Illegal BsaI.rc site found at 2502
    Illegal BsaI.rc site found at 2553