Difference between revisions of "Part:BBa K2729013"
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The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
 
The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
  
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[[File:Tokyotech 2018 EP (E,prME).png.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
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[[File:Tokyotech 2018 EP (E,prME).png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
  
 
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Revision as of 01:14, 17 October 2018


DENV4 ancC-prM-E (Dengue Virus Serotype IV)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Figure 1: Result of the Electrophoresis


As shown in Figure 1, the band of DENV4 prME come around 2,000 bp.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal BamHI site found at 1910
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
    Illegal NgoMIV site found at 1207
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 793

Reference

- Membranotropic regions of the dengue virus prM protein