Difference between revisions of "Part:BBa K2812006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | DNA sequence codon optimized for E. coli. | + | Original DNA sequence based on ''Pseudomonas aeruginosa'' PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985). This DNA sequence is codon optimized for E. coli. |
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===Source=== | ===Source=== | ||
Revision as of 00:55, 17 October 2018
Coding sequence for Pyocin S5 with HlyA and His6-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
Illegal BglII site found at 425
Illegal BglII site found at 2110 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1200
Design Notes
Original DNA sequence based on Pseudomonas aeruginosa PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985). This DNA sequence is codon optimized for E. coli.
Source
Genomic sequence from Pseudomonas aeruginosa. DNA construct produced by IDT.
References
Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.