Difference between revisions of "Part:BBa K575024"

Revision as of 00:25, 17 October 2018

This construct was developed by Northwestern's 2011 iGEM team as part of a Pseudomonas Aeruginosa detector. The device is designed to fluoresce with GFP in the presence of PAI1 (3-oxo-C12-HSL), one of the Pseudomonas quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI1 from the environment and the LasR receptor (produced by this construct).

LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0030), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 58
Illegal NheI site found at 921
Illegal NheI site found at 944
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1300
Illegal AgeI site found at 1497
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 830

[http://2018.igem.org/Team:RMHS_Maryland Team RMHS_Maryland 2018] improved this part by obtaining a novel dose-dependent expression data for this construct in a different chassis, E. coli BL21, at realistic AI-1 concentrations (5-1000nM) that E. coli produce and respond to, demonstrating the construct’s suitability for use in co-culture.
(--ishayardi 20:24, 16 October 2018 (UTC))

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-[http://2018.igem.org/Team:RMHS_Maryland] improved this part by obtaining a novel dose-dependent expression data for this construct in a different chassis, E. coli BL21, at realistic AI-1 concentrations (5-1000nM) that E. coli produce and respond to, demonstrating the construct’s suitability for use in co-culture.
+[http://2018.igem.org/Team:RMHS_Maryland Team RMHS_Maryland 2018] improved this part by obtaining a novel dose-dependent expression data for this construct in a different chassis, E. coli BL21, at realistic AI-1 concentrations (5-1000nM) that E. coli produce and respond to, demonstrating the construct’s suitability for use in co-culture.
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 (<small>--[[User:ishayardi|ishayardi]] 20:24, 16 October 2018 (UTC)</small>)