Difference between revisions of "Part:BBa J72001"

Latest revision as of 05:04, 16 July 2008

{FRT} recombination site for flp recombinase in BBb

DNA substate for the flp recombinase. This part has been successfully used to construct a variety of excisable genomic knockin/knockout cassettes. By transforming a cell with helper plasmid pCP20 (see Datsenko/Wanner paper), cassettes flanked by FRT sites are excised by recombination. Part is referred to internally as Bca1115.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 18
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 18
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 18
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_J72001 short</partinfo>
-Later
+DNA substate for the flp recombinase.  This part has been successfully used to construct a variety of excisable genomic knockin/knockout cassettes.  By transforming a cell with helper plasmid pCP20 (see Datsenko/Wanner paper), cassettes flanked by FRT sites are excised by recombination.  Part is referred to internally as Bca1115.
+----
+This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:<br>
+[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
 <!-- Add more about the biology of this part here