Difference between revisions of "Part:BBa K1469002"
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<partinfo>BBa_K1469002 parameters</partinfo>
 
<partinfo>BBa_K1469002 parameters</partinfo>
 
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=====Improvement=====
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2018 SSTi-SZGD coexpress tuaD and gtaB together, because gtab and tuad are two of the genes involved in the synthesis of UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase. The results showed that co-expressing of tuaD and gtaB genes increased HA accumulation, suggesting that UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase, which commit to the biosynthesis of one of the HA precursors, are rate-limiting enzymes in HA biosynthesis.

Revision as of 23:15, 16 October 2018

UDP-GlcDH

UDP-glucose 6-dehydrogenase of Bacillus megaterium. The enzyme catalyses the NAD dependent oxidation of UDP-glucose to UDP-glucuronic acid, a key precursor molecule for hyaluronic acid production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Improvement

2018 SSTi-SZGD coexpress tuaD and gtaB together, because gtab and tuad are two of the genes involved in the synthesis of UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase. The results showed that co-expressing of tuaD and gtaB genes increased HA accumulation, suggesting that UDP-glucose dehydrogenase and UDP-gulcose pyrophosphorylase, which commit to the biosynthesis of one of the HA precursors, are rate-limiting enzymes in HA biosynthesis.