Difference between revisions of "Part:BBa K2560031:Design"

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===Design Notes===
 
===Design Notes===
 
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The sequence was obtained from part <a href="https://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning.
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The sequence was obtained from part <a href="https://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>. The parts sequence was not changed apart from adding the promoter overhangs that are required for subsequent cloning.
 
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===Source===
 
===Source===
  
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More information coming soon!
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===References===
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
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<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.
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<b> Forward Oligo:</b>
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CTCGGGAGTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACT
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<b> Reverse Oligo:</b>
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CTCAAGTAGCTAGCATTATACCTAGGACTGAGCTAGCTGTCAACTCC

Latest revision as of 22:41, 16 October 2018


Phytobrick version of BBa_J23119


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was obtained from part BBa_J23119. The parts sequence was not changed apart from adding the promoter overhangs that are required for subsequent cloning.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGGGAGTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACT

Reverse Oligo: CTCAAGTAGCTAGCATTATACCTAGGACTGAGCTAGCTGTCAACTCC