Difference between revisions of "Part:BBa K2812003:Design"

 
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===Design Notes===
 
===Design Notes===
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DNA sequence codon optimized for E. coli.  
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The initial starting sequence for truncated lysostaphin was taken from <partinfo>BBa_K748002</partinfo>. This amino acid sequence was used to generate a codon optimized sequence for expression in E. coli.
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Source
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The designed sequence was synthesized ''de novo by'' IDT.
 
===Source===
 
===Source===
  

Revision as of 21:53, 16 October 2018


Coding sequence for trunctated Lysostaphin regulated by T7-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial starting sequence for truncated lysostaphin was taken from BBa_K748002. This amino acid sequence was used to generate a codon optimized sequence for expression in E. coli.

Source The designed sequence was synthesized de novo by IDT.

Source

Uniprot

References