Difference between revisions of "Part:BBa K2680265:Design"
(→Design Notes) |
|||
| (2 intermediate revisions by 2 users not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| + | |||
Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary. | Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary. | ||
===Source=== | ===Source=== | ||
| − | + | Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. sfCFP is a variant of GFP, originally from Aequorea victoria. | |
| − | + | ||
| − | + | ||
Latest revision as of 21:04, 16 October 2018
3G sfCFP-pdt3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 47
Illegal BsaI.rc site found at 861
Illegal SapI.rc site found at 67
Design Notes
Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.
Source
Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. sfCFP is a variant of GFP, originally from Aequorea victoria.