Difference between revisions of "Part:BBa K2637049:Design"

 
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===Design Notes===
 
===Design Notes===
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We added the constitutive <i>TDH3</i> promoter and <i>ADH1</i> terminator to the [https://parts.igem.org/Part:BBa_E2030 Part:BBa_E2030] to make the cassette which can express EYFP continously.
  
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===Source===
  
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The gene <i>EYFP</i> derives from [https://parts.igem.org/Part:BBa_E2030 Part:BBa_E2030]. The <i>TDH3</i> promoter and <i>ADH1</i> terminator are from <i>saccharomyces cerevisiae</i>.
  
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===Source===
 
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===References===
 
===References===

Latest revision as of 21:00, 16 October 2018


EYFP cassette with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1717


Design Notes

We added the constitutive TDH3 promoter and ADH1 terminator to the Part:BBa_E2030 to make the cassette which can express EYFP continously.

Source

The gene EYFP derives from Part:BBa_E2030. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.


References