Difference between revisions of "Part:BBa K209023"
Line 22: Line 22:
  
 
First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.
 
First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.
 +
 
https://parts.igem.org/Part:BBa_K2601008
 
https://parts.igem.org/Part:BBa_K2601008
  
 
Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
 
Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
  
−
href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601025
+
https://parts.igem.org/Part:BBa_K2601007
−
href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601026
+
https://parts.igem.org/Part:BBa_K2601007
−
href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601027
+
https://parts.igem.org/Part:BBa_K2601007
  
 
What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
 
What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
−
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601011</a><br/>
+
https://parts.igem.org/Part:BBa_K2601007
  
 
Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
 
Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
−
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601037</a><br/>
+
https://parts.igem.org/Part:BBa_K2601007
−
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601038</a><br/>
+
https://parts.igem.org/Part:BBa_K2601007

Revision as of 19:37, 16 October 2018


Aar1 C-D part, FKBP

dimerizes with FRB when rapamycin is present

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2018 Peking Team improvement

Team Peking 2018 improved this part.

First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601008

Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.

https://parts.igem.org/Part:BBa_K2601007 https://parts.igem.org/Part:BBa_K2601007 https://parts.igem.org/Part:BBa_K2601007

What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin. https://parts.igem.org/Part:BBa_K2601007

Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry. https://parts.igem.org/Part:BBa_K2601007 https://parts.igem.org/Part:BBa_K2601007