Difference between revisions of "Part:BBa K209023"
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First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope. | First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope. | ||
| − | https://parts.igem.org/Part: | + | https://parts.igem.org/Part:BBa_K2601008 |
Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry. | Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry. | ||
Revision as of 19:37, 16 October 2018
Aar1 C-D part, FKBP
dimerizes with FRB when rapamycin is present
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2018 Peking Team improvement
Team Peking 2018 improved this part.
First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope. https://parts.igem.org/Part:BBa_K2601008
Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601025 href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601026 href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601027
What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601011</a>
Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601037</a>
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601038</a>