Difference between revisions of "Part:BBa K2764000"

Latest revision as of 17:01, 16 October 2018

Alkane channel protein

The alkL protein is an alkane channel protein that comes from Pseudomonas Oleovorans. Its function is mainly to import medium to long chain alkanes into the cell. In Gram-negative cells, such as Escherichia coli and Shewanella oneidensis, import of medium to long chain alkanes is inhibited by the cell's lippopolysaccharide layer. The alkL protein's extracellular domain penetrates this layer and binds to alkane molecules with high affinity. Once the alkane has been bound, it is transported through the hydrophobic core of the alkL protein, thus bypassing the lipopolysaccharide layer. The alkane then enters the periplasm of the cell, where it can then diffuse into the cytoplasm. According to literature, the alkL protein is toxic when overexpressed and may inhibit host cell growth.

References:
1. Grant, C., Deszcz, D., Wei, Y., Martínez-Torres, R., Morris, P., Folliard, T., Sreenivasan, R., Ward, J., Dalby, P., Woodley, J. and Baganz, F. (2014). Identification and use of an alkane transporter plug-in for applications in biocatalysis and whole-cell biosensing of alkanes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 651
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 303
Illegal NgoMIV site found at 516
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2764000 short</partinfo>
-The alkL protein is an alkane channel protein that comes from <i>Pseudomonas Oleovorans.</i> Its function is mainly to import medium to long chain alkanes into the cell. In Gram-negative cells, such as <i>Escherichia coli</i> and <i>Shewanella oneidensis</i>, import of medium to long chain alkanes is inhibited by the cell's lippopolysaccharide layer. The alkL protein's extracellular domain penetrates this layer and binds to alkane molecules with high affinity. Once the alkane has been bound, it is transported through the hydrophobic core of the alkL protein, thus bypassing the lipopolysaccharide layer, and enters the periplasm of the cell, where it can then diffuse into the cytoplasm.
+The alkL protein is an alkane channel protein that comes from <i>Pseudomonas Oleovorans.</i> Its function is mainly to import medium to long chain alkanes into the cell. In Gram-negative cells, such as <i>Escherichia coli</i> and <i>Shewanella oneidensis</i>, import of medium to long chain alkanes is inhibited by the cell's lippopolysaccharide layer. The alkL protein's extracellular domain penetrates this layer and binds to alkane molecules with high affinity. Once the alkane has been bound, it is transported through the hydrophobic core of the alkL protein, thus bypassing the lipopolysaccharide layer. The alkane then enters the periplasm of the cell, where it can then diffuse into the cytoplasm. According to literature, the alkL protein is toxic when overexpressed and may inhibit host cell growth.
+https://static.igem.org/mediawiki/2018/d/d1/T--Hong_Kong_HKUST--alklchannelnew.png
+<p>References: <br>
+. Grant, C., Deszcz, D., Wei, Y., Martínez-Torres, R., Morris, P., Folliard, T., Sreenivasan, R., Ward, J., Dalby, P., Woodley, J. and Baganz, F. (2014). Identification and use of an alkane transporter plug-in for applications in biocatalysis and whole-cell biosensing of alkanes. </p>
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