Difference between revisions of "Part:BBa K2680280:Design"

 
 
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===Design Notes===
 
===Design Notes===
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To do
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TlpA39 is a mutated form of TlpA, sequence from Piraner et. al 2017.<sup>1</sup>
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===Source===
 
===Source===
  
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To do
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TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression <sup>1</sup> Original sequence: Piraner, Dan I., et al.
  
 
===References===
 
===References===
 +
1. Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233

Latest revision as of 16:11, 16 October 2018


3G TlpA39


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1170


Design Notes

TlpA39 is a mutated form of TlpA, sequence from Piraner et. al 2017.1


Source

TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression 1 Original sequence: Piraner, Dan I., et al.

References

1. Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233