Difference between revisions of "Part:BBa K2680280:Design"
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | TlpA39 is a mutated form of TlpA, sequence from Piraner et. al 2017.<sup>1</sup> | |
Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
− | + | TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression <sup>1</sup> Original sequence: Piraner, Dan I., et al. | |
===References=== | ===References=== | ||
+ | 1. Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233 |
Revision as of 16:11, 16 October 2018
3G TlpA39
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 556
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 841
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1144
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI.rc site found at 1170
Design Notes
TlpA39 is a mutated form of TlpA, sequence from Piraner et. al 2017.1
Source
TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression 1 Original sequence: Piraner, Dan I., et al.
References
1. Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233