Difference between revisions of "Part:BBa K2680539:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Sequence was taken from a publication from a lab at the California Institute of Technology. <sup>1</sup> | + | Sequence was taken from a publication from a lab at the California Institute of Technology.Forbidden restriction sites were checked for and removed if necessary. <sup>1</sup> |
===Source=== | ===Source=== | ||
Organism: <i>Salmonella typhimurium</i><br> | Organism: <i>Salmonella typhimurium</i><br> | ||
− | Original sequence: Piraner, Dan I., et al. | + | TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression <sup>1</sup> |
− | + | Original sequence: Piraner, Dan I., et al. | |
===References=== | ===References=== | ||
+ | 1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80. |
Latest revision as of 15:53, 16 October 2018
TlpA39
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 508
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 793
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1096
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was taken from a publication from a lab at the California Institute of Technology.Forbidden restriction sites were checked for and removed if necessary. 1
Source
Organism: Salmonella typhimurium
TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression 1
Original sequence: Piraner, Dan I., et al.
References
1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.