Difference between revisions of "Part:BBa K2680539:Design"

(→‎Design Notes)
(→‎Source)
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Organism: <i>Salmonella typhimurium</i><br>
 
Organism: <i>Salmonella typhimurium</i><br>
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Original sequence: Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.<br>
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"orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression <sub>1</sub>
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Source of the sequence: IDT oligo
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Original sequence: Piraner, Dan I., et al.
  
 
===References===
 
===References===
 
1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.
 
1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.

Revision as of 15:52, 16 October 2018


TlpA39


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was taken from a publication from a lab at the California Institute of Technology.Forbidden restriction sites were checked for and removed if necessary. 1

Source

Organism: Salmonella typhimurium
"orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression 1 Original sequence: Piraner, Dan I., et al.

References

1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.