Difference between revisions of "Part:BBa K2680280"
Line 8: | Line 8: | ||
===References=== | ===References=== | ||
+ | |||
1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80. | 1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80. | ||
Revision as of 15:43, 16 October 2018
3G TlpA39
3G TlpA39 is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
3G TlpA39 is a a temperature sensitive repressor mtuated from Tlpa. Tlpa is a " tunable, orthogonal, temperature-dependent transcriptional repressor providing switch-like control of bacterial gene expression at thresholds spanning the biomedically relevant range of 32-46 °C" 1 Tlpa39 is mutated so that its derepression occurs at a higher temperature, 39 C
===References===
1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 556
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 841
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1144
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI.rc site found at 1170