Difference between revisions of "Part:BBa K2680539:Design"

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===Design Notes===
 
===Design Notes===
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Sequence was taken from a publication from a lab at the California Institute of Technology, and was checked for illegal restriction sites. <sup>1</sup>
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Sequence was taken from a publication from a lab at the California Institute of Technology.Forbidden restriction sites were checked for and removed if necessary. <sup>1</sup>
  
 
===Source===
 
===Source===

Revision as of 15:37, 16 October 2018


TlpA39


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was taken from a publication from a lab at the California Institute of Technology.Forbidden restriction sites were checked for and removed if necessary. 1

Source

Organism: Salmonella typhimurium
Original sequence: Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.
Source of the sequence: IDT oligo

References

1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.