Difference between revisions of "Part:BBa K2729009"
Line 5: | Line 5: | ||
AmCyan is one of the existing violet fluorochromes for use in flow cytometers equipped with a violet (405 nm) laser. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron. | AmCyan is one of the existing violet fluorochromes for use in flow cytometers equipped with a violet (405 nm) laser. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron. | ||
− | + | ===Characterization=== | |
− | === | + | |
− | < | + | The sequence is optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it. |
− | < | + | |
− | + | ||
+ | [[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|300px| '''Figure 1:''' '''Result of the qualitative experiment''' - description comes here. ]] | ||
− | < | + | <br> |
− | === | + | As shown in Figure 1, the band comes between 900 bp to 1,000 bp. |
− | <partinfo>BBa_K2729009 | + | <br style="clear: both" /> |
− | + | ||
+ | ===Analysis=== | ||
+ | |||
+ | Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection. | ||
+ | |||
+ | ===Sequence and Features=== | ||
+ | <partinfo>BBa_K2729009 SequenceAndFeatures</partinfo> |
Revision as of 14:31, 16 October 2018
AmCyan_FMDV2a
AmCyan is one of the existing violet fluorochromes for use in flow cytometers equipped with a violet (405 nm) laser. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
Characterization
The sequence is optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
As shown in Figure 1, the band comes between 900 bp to 1,000 bp.
Analysis
Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 43
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 820
Illegal AgeI site found at 669 - 1000COMPATIBLE WITH RFC[1000]