Difference between revisions of "Part:BBa K2729008"

 
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<partinfo>BBa_K2729008 short</partinfo>
 
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YFP is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
 
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===Usage and Biology===
 
===Usage and Biology===
  
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ZsYellow is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2729008 SequenceAndFeatures</partinfo>
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===Characterization===
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The sequence is optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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[[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|300px| '''Figure 1:''' '''Result of the qualitative experiment''' - description comes here. ]]
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As shown in Figure 1, the band comes between 900 bp to 1,000 bp.
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===Analysis===
  
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
  
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===Sequence and Features===
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===Functional Parameters===
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<partinfo>BBa_K2729006 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2729008 parameters</partinfo>
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Revision as of 14:30, 16 October 2018


ZsYellow-FMDV 2A

Usage and Biology

ZsYellow is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.

Characterization

The sequence is optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the qualitative experiment - description comes here.


As shown in Figure 1, the band comes between 900 bp to 1,000 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]