Difference between revisions of "Part:BBa K2718011"
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We producted HmaS in ''E.coli'' DH5alpha, with 1mm IPTG at OD 0.8, 3 hours | We producted HmaS in ''E.coli'' DH5alpha, with 1mm IPTG at OD 0.8, 3 hours | ||
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| + | Figure 2 SDS-PAGE of HmaS production with induction (2, 3 and) 4, without induction ( 1,NI) and with empty plasmid (5) | ||
Revision as of 14:23, 16 October 2018
IPTG inductible promotor RBS (strong) HmaS
Usage and Biology
HmaS is used in the metabolic pathway shown in figure 1 to produce benzyl alcohol. HmaS catalyzes the oxidative decarboxylation of phenylpyruvate to produce (S)Mandelate.
Figure 1 Metabolic pathway to produce benzyl alcohol
Production
We producted HmaS in E.coli DH5alpha, with 1mm IPTG at OD 0.8, 3 hours
Figure 2 SDS-PAGE of HmaS production with induction (2, 3 and) 4, without induction ( 1,NI) and with empty plasmid (5)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 607
Illegal BamHI site found at 716 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 556