Difference between revisions of "Part:BBa K2548013"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2548013 short</partinfo> | <partinfo>BBa_K2548013 short</partinfo> | ||
| − | This | + | This design is used to synthesize and extract distinct scydonadin in order to test its effectiveness. We chose the pTac promoter, a hybrid of Lac and Trp promoters, to induce scydonadin production in E. coli because it is the most accessible promoter in our lab. The promoter can be induced by IPTG to remove the repression on transcription from LacI. We also add 6xHis-tag after sequences of sydonadin to extract it out with the nickel column. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:09, 16 October 2018
LacI - pTac - Scygonadin + 6xHis Tag - Terminator
This design is used to synthesize and extract distinct scydonadin in order to test its effectiveness. We chose the pTac promoter, a hybrid of Lac and Trp promoters, to induce scydonadin production in E. coli because it is the most accessible promoter in our lab. The promoter can be induced by IPTG to remove the repression on transcription from LacI. We also add 6xHis-tag after sequences of sydonadin to extract it out with the nickel column.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1442
- 1000COMPATIBLE WITH RFC[1000]